Protein Properties
Description 14-3-3 Protein Gf14 Phi (Grf4)
Comment identical to GF14 protein phi chain GI:1493805, SP:P46077 from (Arabidopsis thaliana)
Classification Eukaryotes
TIGR ID At1g35160
GB AC079605
Gene Family based on BLASTCLUST Family Size: 14
Expert gene family (data source: TAIR)
(Super Gene Family / Gene Family)		 Ca-binding proteins / 14-3-3s; NA / 14-3-3 proteins
MW (TIGR) 30193.84
pI (TIGR) 4.5036
EC Analysis (KEGG) N/A
External Links [TIGR]  [MIPS]  [TAIR]  [SIGnAL]

Subcellular Localization (TargetP Prediction)
Localization Chloroplast
Score		 Mitochondrial
Score		 Secretory
Score		 Signal Length Reliability Class
Not Defined .125 .047 .358 N/A 4

Gene Expression in different plant organs
EST data
TC Total # ESTs Seeds Roots Leaves Flowers Siliques Seedlings Cell Suspension Mixed Tissues
TC160711 45 0 (0.00%) 10 (0.05%) 0 (0.00%) 1 (0.01%) 11 (0.09%) 3 (0.05%) 1 (0.10%) 5 (0.01%)
AFGC microarray data (Log2 of normalized ratio)
Flower vs. Leaves Stem vs. Ref. Silique vs. Ref. Root vs. Ref. Flower vs. Ref. Leaf vs. Ref.
.6605 .426 .229 .2225 .079 -.394

Membrane/Soluble (TMHMM Prediction)
Location Expected # of AA Expected # of AA in first 60 Predicted Helices Topology
soluble .02 0 0 o

MIPS Automatic Functional Classification
Category Number Description Hitcode P-value
10.01 Intracellular Signalling IPI00000816 1e-125
63.01 Protein Binding IPI00000816 1e-125
10.01.01 Unspecified Signal Transduction At4g09000 1e-124
20.25.01 Animal Sensory Mechanisms c8001086 1e-118
62.01.07 Binding / Dissociation c8001086 1e-118
63.17.01 Calcium Binding c8001086 1e-118
13.11.03.05 Chemical Environment Response c8001086 1e-118
40.03 Cytoplasm c8001086 1e-118
20.25.07.03 Effect On Homeostasis (E.G. Metabolism Or Electrolyte Homeostasis) c8001086 1e-118
62.02.01 Enzymatic Activity Regulation / Enzyme Regulator c8001086 1e-118
63.13.03 Fatty Acid Binding (Eg Acyl-Carrier Protein) c8001086 1e-118
13.01.01.01 Homeostasis Of Metal Ions (Na, K, Ca Etc.) c8001086 1e-118
62.01.01 Modification c8001086 1e-118
43.03.13 Neuron c8001086 1e-118
43.03.99 Other Animal Cell Type Localisation c8001086 1e-118
20.25.07.99 Other Hormonal Response Activity c8001086 1e-118
63.02 Peptide Binding c8001086 1e-118
62.02.07 Regulator Of Receptor Activity c8001086 1e-118

Gene Ontology
Accession Description Type Code Evidence Reference Synonym
GO:0005635 nuclear membrane component TAS TAIR:Publication:501681748 nuclear envelope
GO:0005737 cytoplasm component TAS TAIR:Publication:501681748
GO:0005886 plasma membrane component TAS TAIR:Publication:501681748 cell membrane
GO:0005886 plasma membrane component TAS TAIR:Publication:501681748 cytoplasmic membrane
GO:0007165 signal transduction process TAS PMID:10631267

Protein Domain Analysis
Accession Description Score
No HMM data available

Signal-P Analysis
Prediction SA Probability SP Probability Cleavage Prob. Position
Non-Secretory Protein 0.000 0.002 0.001 26