Protein Properties
Description 14-3-3 Protein Gf14 Chi (Grf1)
Comment identical to 14-3-3 protein GF14 chi chain GI:1702986, SP:P42643 from (Arabidopsis thaliana)
Classification Eukaryotes
TIGR ID At4g09000
GB AC005359
Gene Family based on BLASTCLUST Family Size: 14
Expert gene family (data source: TAIR)
(Super Gene Family / Gene Family)		 Ca-binding proteins / 14-3-3s; NA / 14-3-3 proteins
MW (TIGR) 29931.51
pI (TIGR) 4.4235
EC Analysis (KEGG) N/A
External Links [TIGR]  [MIPS]  [TAIR]  [SIGnAL]

Subcellular Localization (TargetP Prediction)
Localization Chloroplast
Score		 Mitochondrial
Score		 Secretory
Score		 Signal Length Reliability Class
Not Defined .064 .065 .3 N/A 3

Gene Expression in different plant organs
EST data
TC Total # ESTs Seeds Roots Leaves Flowers Siliques Seedlings Cell Suspension Mixed Tissues
No EST Breakdown Available
AFGC microarray data (Log2 of normalized ratio)
Flower vs. Leaves Stem vs. Ref. Silique vs. Ref. Root vs. Ref. Flower vs. Ref. Leaf vs. Ref.
.323 .2535 .036 -.082 -.2355 -.3925

Membrane/Soluble (TMHMM Prediction)
Location Expected # of AA Expected # of AA in first 60 Predicted Helices Topology
soluble .02 0 0 o

MIPS Automatic Functional Classification
Category Number Description Hitcode P-value
10.01.01 Unspecified Signal Transduction At4g09000 1e-124
20.25.01 Animal Sensory Mechanisms c8001086 1e-121
62.01.07 Binding / Dissociation c8001086 1e-121
63.17.01 Calcium Binding c8001086 1e-121
13.11.03.05 Chemical Environment Response c8001086 1e-121
40.03 Cytoplasm c8001086 1e-121
20.25.07.03 Effect On Homeostasis (E.G. Metabolism Or Electrolyte Homeostasis) c8001086 1e-121
62.02.01 Enzymatic Activity Regulation / Enzyme Regulator c8001086 1e-121
63.13.03 Fatty Acid Binding (Eg Acyl-Carrier Protein) c8001086 1e-121
13.01.01.01 Homeostasis Of Metal Ions (Na, K, Ca Etc.) c8001086 1e-121
10.05.02.30 Ion Channel Mediated Signalling Pathway c8001086 1e-121
62.01.01 Modification c8001086 1e-121
43.03.13 Neuron c8001086 1e-121
43.03.99 Other Animal Cell Type Localisation c8001086 1e-121
20.25.07.99 Other Hormonal Response Activity c8001086 1e-121
10.05.01.12.99 Other Transmembrane Receptor Protein Tyrosine Kinase Signalling Pathways c8001086 1e-121
63.02 Peptide Binding c8001086 1e-121
63.01 Protein Binding c8001086 1e-121
62.02.07 Regulator Of Receptor Activity c8001086 1e-121

Gene Ontology
Accession Description Type Code Evidence Reference Synonym
GO:0005634 nucleus component IDA TAIR:Publication:501681748
GO:0007165 signal transduction process TAS PMID:10631267

Protein Domain Analysis
Accession Description Score
PF00244 14-3-3 protein 512.1

Signal-P Analysis
Prediction SA Probability SP Probability Cleavage Prob. Position
Non-Secretory Protein 0.000 0.000 0.000 25