Protein Properties
Description 14-3-3 Protein Gf14 Lambda (Grf6/Aft1)
Comment identical to 14-3-3 GF14lambda GI:1345595 from (Arabidopsis thaliana)
Classification Eukaryotes
TIGR ID At5g10450
GB AL353995
Gene Family based on BLASTCLUST Family Size: 14
Expert gene family (data source: TAIR)
(Super Gene Family / Gene Family)		 NA / 14-3-3 proteins
MW (TIGR) 27975.58
pI (TIGR) 4.4877
EC Analysis (KEGG) N/A
External Links [TIGR]  [MIPS]  [TAIR]  [SIGnAL]

Subcellular Localization (TargetP Prediction)
Localization Chloroplast
Score		 Mitochondrial
Score		 Secretory
Score		 Signal Length Reliability Class
Not Defined .047 .196 .173 N/A 3

Gene Expression in different plant organs
EST data
TC Total # ESTs Seeds Roots Leaves Flowers Siliques Seedlings Cell Suspension Mixed Tissues
TC160713 111 4 (0.04%) 4 (0.02%) 0 (0.00%) 1 (0.01%) 18 (0.14%) 3 (0.05%) 0 (0.00%) 46 (0.11%)
AFGC microarray data (Log2 of normalized ratio)
Flower vs. Leaves Stem vs. Ref. Silique vs. Ref. Root vs. Ref. Flower vs. Ref. Leaf vs. Ref.
.3455 .32225 .16075 .037 -.11475 -.20575

Membrane/Soluble (TMHMM Prediction)
Location Expected # of AA Expected # of AA in first 60 Predicted Helices Topology
soluble .13 0 0 o

MIPS Automatic Functional Classification
Category Number Description Hitcode P-value
20.25.01 Animal Sensory Mechanisms c8001086 1e-114
62.01.07 Binding / Dissociation c8001086 1e-114
63.17.01 Calcium Binding c8001086 1e-114
13.11.03.05 Chemical Environment Response c8001086 1e-114
40.03 Cytoplasm c8001086 1e-114
20.25.07.03 Effect On Homeostasis (E.G. Metabolism Or Electrolyte Homeostasis) c8001086 1e-114
62.02.01 Enzymatic Activity Regulation / Enzyme Regulator c8001086 1e-114
63.13.03 Fatty Acid Binding (Eg Acyl-Carrier Protein) c8001086 1e-114
13.01.01.01 Homeostasis Of Metal Ions (Na, K, Ca Etc.) c8001086 1e-114
10.05.02.30 Ion Channel Mediated Signalling Pathway c8001086 1e-114
62.01.01 Modification c8001086 1e-114
43.03.13 Neuron c8001086 1e-114
43.03.99 Other Animal Cell Type Localisation c8001086 1e-114
20.25.07.99 Other Hormonal Response Activity c8001086 1e-114
10.05.01.12.99 Other Transmembrane Receptor Protein Tyrosine Kinase Signalling Pathways c8001086 1e-114
63.02 Peptide Binding c8001086 1e-114
63.01 Protein Binding c8001086 1e-114
62.02.07 Regulator Of Receptor Activity c8001086 1e-114
10.05.01.18 Transmembrane Receptor Protein Serine/Threonine Kinase Signalling Pathways c8001086 1e-114

Gene Ontology
Accession Description Type Code Evidence Reference Synonym
GO:0005634 nucleus component IDA TAIR:Publication:501681748
GO:0005737 cytoplasm component IDA TAIR:Publication:501681748
GO:0005886 plasma membrane component IDA TAIR:Publication:501681748 cell membrane
GO:0005886 plasma membrane component IDA TAIR:Publication:501681748 cytoplasmic membrane
GO:0007165 signal transduction process TAS PMID:10631267

Protein Domain Analysis
Accession Description Score
No HMM data available

Signal-P Analysis
Prediction SA Probability SP Probability Cleavage Prob. Position
Non-Secretory Protein 0.000 0.000 0.000 22