Protein Properties
Description 14-3-3 Protein Gf14 Kappa (Grf8)
Comment identical to 14-3-3 protein GF14 kappa GI:5802794, SP:P48348 from (Arabidopsis thaliana)
Classification Eukaryotes
TIGR ID At5g65430
GB AB011479
Gene Family based on BLASTCLUST Family Size: 14
Expert gene family (data source: TAIR)
(Super Gene Family / Gene Family)		 Ca-binding proteins / 14-3-3s; NA / 14-3-3 proteins
MW (TIGR) 28028.65
pI (TIGR) 4.5365
EC Analysis (KEGG) N/A
External Links [TIGR]  [MIPS]  [TAIR]  [SIGnAL]

Subcellular Localization (TargetP Prediction)
Localization Chloroplast
Score		 Mitochondrial
Score		 Secretory
Score		 Signal Length Reliability Class
Not Defined .052 .182 .141 N/A 3

Gene Expression in different plant organs
EST data
TC Total # ESTs Seeds Roots Leaves Flowers Siliques Seedlings Cell Suspension Mixed Tissues
TC160712 43 1 (0.01%) 2 (0.01%) 2 (0.05%) 1 (0.01%) 10 (0.08%) 4 (0.06%) 0 (0.00%) 11 (0.03%)
AFGC microarray data (Log2 of normalized ratio)
Flower vs. Leaves Stem vs. Ref. Silique vs. Ref. Root vs. Ref. Flower vs. Ref. Leaf vs. Ref.
.558 .274 -.031 -.2115 -.437 -.513

Membrane/Soluble (TMHMM Prediction)
Location Expected # of AA Expected # of AA in first 60 Predicted Helices Topology
soluble .05 0 0 o

MIPS Automatic Functional Classification
Category Number Description Hitcode P-value
20.25.01 Animal Sensory Mechanisms c8001086 1e-118
62.01.07 Binding / Dissociation c8001086 1e-118
63.17.01 Calcium Binding c8001086 1e-118
13.11.03.05 Chemical Environment Response c8001086 1e-118
40.03 Cytoplasm c8001086 1e-118
20.25.07.03 Effect On Homeostasis (E.G. Metabolism Or Electrolyte Homeostasis) c8001086 1e-118
62.02.01 Enzymatic Activity Regulation / Enzyme Regulator c8001086 1e-118
63.13.03 Fatty Acid Binding (Eg Acyl-Carrier Protein) c8001086 1e-118
13.01.01.01 Homeostasis Of Metal Ions (Na, K, Ca Etc.) c8001086 1e-118
10.05.02.30 Ion Channel Mediated Signalling Pathway c8001086 1e-118
62.01.01 Modification c8001086 1e-118
43.03.13 Neuron c8001086 1e-118
43.03.99 Other Animal Cell Type Localisation c8001086 1e-118
20.25.07.99 Other Hormonal Response Activity c8001086 1e-118
10.05.01.12.99 Other Transmembrane Receptor Protein Tyrosine Kinase Signalling Pathways c8001086 1e-118
63.02 Peptide Binding c8001086 1e-118
63.01 Protein Binding c8001086 1e-118
62.02.07 Regulator Of Receptor Activity c8001086 1e-118
10.05.01.18 Transmembrane Receptor Protein Serine/Threonine Kinase Signalling Pathways c8001086 1e-118

Gene Ontology
Accession Description Type Code Evidence Reference Synonym
GO:0005634 nucleus component IDA TAIR:Publication:501681748
GO:0005737 cytoplasm component IDA TAIR:Publication:501681748
GO:0005886 plasma membrane component IDA TAIR:Publication:501681748 cell membrane
GO:0005886 plasma membrane component IDA TAIR:Publication:501681748 cytoplasmic membrane
GO:0007165 signal transduction process TAS PMID:10631267
GO:0009505 cell wall (sensu Magnoliophyta) component IDA TAIR:Publication:501681748

Protein Domain Analysis
Accession Description Score
PF00244 14-3-3 protein 496.3

Signal-P Analysis
Prediction SA Probability SP Probability Cleavage Prob. Position
Non-Secretory Protein 0.000 0.000 0.000 22