Protein Properties
Description 3-Hydroxyisobutyryl-Coenzyme A Hydrolase (Coa-Thioester Hydrolase) (Chy1)
Comment identical to gi:8572760; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein
Classification Common To All
TIGR ID At5g65940
GB AB018108
Gene Family based on BLASTCLUST Family Size: 6
Expert gene family (data source: TAIR)
(Super Gene Family / Gene Family)		 Acyl-lipid metabolism / Hydroxyisobutyryl-CoA Hydrolase; Membrane proteins / 3-hydroxyisobutyryl-coenzyme A hydrolase family
MW (TIGR) 42073.37
pI (TIGR) 8.2684
EC Analysis (KEGG) N/A
External Links [TIGR]  [MIPS]  [TAIR]  [SIGnAL]

Subcellular Localization (TargetP Prediction)
Localization Chloroplast
Score		 Mitochondrial
Score		 Secretory
Score		 Signal Length Reliability Class
Not Defined .196 .077 .163 N/A 3

Gene Expression in different plant organs
EST data
TC Total # ESTs Seeds Roots Leaves Flowers Siliques Seedlings Cell Suspension Mixed Tissues
TC155252 6 0 (0.00%) 2 (0.01%) 0 (0.00%) 1 (0.01%) 0 (0.00%) 0 (0.00%) 0 (0.00%) 0 (0.00%)
AFGC microarray data (Log2 of normalized ratio)
Flower vs. Leaves Stem vs. Ref. Silique vs. Ref. Root vs. Ref. Flower vs. Ref. Leaf vs. Ref.
No microarray data available

Membrane/Soluble (TMHMM Prediction)
Location Expected # of AA Expected # of AA in first 60 Predicted Helices Topology
soluble 2.79 .04 0 o

MIPS Automatic Functional Classification
Category Number Description Hitcode P-value
01.06.01 Lipid, Fatty-Acid And Isoprenoid Biosynthesis At4g31810 1e-118
01 Metabolism IPI00023517 1e-112
25.05 Animal Development F09F7.4 1e-109
14.04 Cell Differentiation F09F7.4 1e-109
01.06.04 Breakdown Of Lipids, Fatty Acids And Isoprenoids At3g60510 1e-108
40.16 Mitochondrion gi_7303265 2e-61

Gene Ontology
Accession Description Type Code Evidence Reference Synonym
GO:0003860 3-hydroxyisobutyryl-CoA hydrolase function IGI PMID:11404361 NCBI_gi:13636047
GO:0005777 peroxisome component ISS PMID:11404361 PMID:2654139
GO:0006574 valine catabolism process IDA PMID:11404361
GO:0006635 fatty acid beta-oxidation process TAS PMID:11404361

Protein Domain Analysis
Accession Description Score
PF00378 enoyl-CoA hydratase/isomerase family protein 75.4

Signal-P Analysis
Prediction SA Probability SP Probability Cleavage Prob. Position
Non-Secretory Protein 0.000 0.000 0.000 18